This is a Validated Antibody Database (VAD) review about human KLF4, based on 29 published articles (read how Labome selects the articles), using KLF4 antibody in all methods. It is aimed to help Labome visitors find the most suited KLF4 antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
KLF4 synonym: EZF; GKLF

Knockout validation
R&D Systems
domestic goat polyclonal
  • western blot knockout validation; human; 1:1000; loading ...; fig 6c
R&D Systems KLF4 antibody (R&D, AF3640) was used in western blot knockout validation on human samples at 1:1000 (fig 6c). Cell Death Dis (2021) ncbi
Abcam
domestic rabbit monoclonal (EPR19590)
  • western blot; human; loading ...; fig 5a
Abcam KLF4 antibody (Abcam, ab215036) was used in western blot on human samples (fig 5a). Int J Mol Sci (2021) ncbi
domestic rabbit monoclonal (EPR19590)
  • immunocytochemistry; mouse; fig 6i
  • western blot; mouse; 1:1000; fig 6e
Abcam KLF4 antibody (Abcam, ab215036) was used in immunocytochemistry on mouse samples (fig 6i) and in western blot on mouse samples at 1:1000 (fig 6e). Aging (Albany NY) (2021) ncbi
domestic rabbit polyclonal
  • western blot; mouse; loading ...; fig 8a
Abcam KLF4 antibody (Abcam, ab106629) was used in western blot on mouse samples (fig 8a). Cell Death Dis (2021) ncbi
domestic rabbit monoclonal (EPR19590)
  • western blot; rat; 1:1500; fig 5f
Abcam KLF4 antibody (Abcam, ab215036) was used in western blot on rat samples at 1:1500 (fig 5f). Cell Death Dis (2021) ncbi
Santa Cruz Biotechnology
mouse monoclonal (B-8)
  • immunocytochemistry; mouse; loading ...; fig s1g
Santa Cruz Biotechnology KLF4 antibody (Santa Cruz, sc-393462) was used in immunocytochemistry on mouse samples (fig s1g). Cell Rep (2018) ncbi
  • western blot; mouse; loading ...; fig 2b
Santa Cruz Biotechnology KLF4 antibody (Santa Cruz, sc-20691) was used in western blot on mouse samples (fig 2b). Mol Neurobiol (2018) ncbi
  • western blot; pigs ; loading ...; fig 2c
Santa Cruz Biotechnology KLF4 antibody (Santa Cruz, sc-20691) was used in western blot on pigs samples (fig 2c). Sci Rep (2017) ncbi
mouse monoclonal (B-8)
  • immunoprecipitation; human; fig 5
In order to determine the necessity for differentiation-dependent viral life cycle by post-transciptional regulation of KLF4 by high-risk human papillomaviruses, Santa Cruz Biotechnology KLF4 antibody (Santa Cruz Biotechnology, SC-393462) was used in immunoprecipitation on human samples (fig 5). PLoS Pathog (2016) ncbi
  • chromatin immunoprecipitation; mouse; loading ...; fig 6f
In order to research the atheroprotective effect of OCT4 in smooth muscle cells, Santa Cruz Biotechnology KLF4 antibody (Santa-Cruz, sc-20691) was used in chromatin immunoprecipitation on mouse samples (fig 6f). Nat Med (2016) ncbi
  • chromatin immunoprecipitation; human; fig 6
Santa Cruz Biotechnology KLF4 antibody (Santa Cruz, 20691) was used in chromatin immunoprecipitation on human samples (fig 6). PLoS Pathog (2015) ncbi
  • ChIP-Seq; mouse; fig 5
Santa Cruz Biotechnology KLF4 antibody (Santa Cruz, sc20691) was used in ChIP-Seq on mouse samples (fig 5). Nat Med (2015) ncbi
mouse monoclonal (F-8)
  • western blot; mouse; loading ...; fig 3d,4a
Santa Cruz Biotechnology KLF4 antibody (Santa Cruz, sc-166238) was used in western blot on mouse samples (fig 3d,4a). J Cell Biochem (2015) ncbi
mouse monoclonal (F-8)
  • western blot; rat; 1:500
Santa Cruz Biotechnology KLF4 antibody (Santa Cruz Technology, sc-166238) was used in western blot on rat samples at 1:500. Age (Dordr) (2014) ncbi
R&D Systems
domestic goat polyclonal
  • western blot knockout validation; human; 1:1000; loading ...; fig 6c
R&D Systems KLF4 antibody (R&D, AF3640) was used in western blot knockout validation on human samples at 1:1000 (fig 6c). Cell Death Dis (2021) ncbi
domestic goat polyclonal
  • immunohistochemistry - paraffin section; mouse; loading ...; fig 8j
R&D Systems KLF4 antibody (R&D Systems, AF3158) was used in immunohistochemistry - paraffin section on mouse samples (fig 8j). Cell Mol Gastroenterol Hepatol (2021) ncbi
domestic goat polyclonal
  • immunohistochemistry - paraffin section; human; 4 ug/ml; loading ...; fig 1c, 5h
  • western blot; human; 1 ug/ml; loading ...; fig 5c
R&D Systems KLF4 antibody (R&D Systems, AF3640) was used in immunohistochemistry - paraffin section on human samples at 4 ug/ml (fig 1c, 5h) and in western blot on human samples at 1 ug/ml (fig 5c). Cell Mol Gastroenterol Hepatol (2021) ncbi
Novus Biologicals
mouse monoclonal (CL5785)
  • immunohistochemistry - paraffin section; human; 1:1000; loading ...; fig 2b
Novus Biologicals KLF4 antibody (Novus, CL5785) was used in immunohistochemistry - paraffin section on human samples at 1:1000 (fig 2b). Front Endocrinol (Lausanne) (2019) ncbi
Cell Signaling Technology
domestic rabbit monoclonal (D1F2)
  • immunoprecipitation; human; loading ...; fig 7d: 7e
  • western blot; human; 1:500; loading ...; fig 7d, 7e
Cell Signaling Technology KLF4 antibody (CST, 12173S) was used in immunoprecipitation on human samples (fig 7d: 7e) and in western blot on human samples at 1:500 (fig 7d, 7e). Cell Death Dis (2021) ncbi
domestic rabbit polyclonal
  • western blot; human; 1:1000; loading ...; fig 4e
Cell Signaling Technology KLF4 antibody (Cell Signaling, 4038S) was used in western blot on human samples at 1:1000 (fig 4e). Neoplasia (2021) ncbi
domestic rabbit polyclonal
  • western blot; human; 1:500; loading ...; fig s5h
Cell Signaling Technology KLF4 antibody (Cell Signaling, 4038) was used in western blot on human samples at 1:500 (fig s5h). Sci Adv (2019) ncbi
domestic rabbit polyclonal
  • immunocytochemistry; human; loading ...; fig 2b
  • western blot; human; 1:1000; loading ...; fig 2a
Cell Signaling Technology KLF4 antibody (Cell signaling, 4038) was used in immunocytochemistry on human samples (fig 2b) and in western blot on human samples at 1:1000 (fig 2a). Front Physiol (2016) ncbi
domestic rabbit polyclonal
  • western blot; mouse; loading ...; fig 4d
In order to study the role of miR-7 in acute lung injury, Cell Signaling Technology KLF4 antibody (CST, 4038) was used in western blot on mouse samples (fig 4d). Front Immunol (2016) ncbi
domestic rabbit monoclonal (D1F2)
  • western blot; human; 1:1000; fig 2
In order to investigate the contribution of DNA methyltransferase 1 to the epithelial-mesenchymal transition and cancer stem cells, Cell Signaling Technology KLF4 antibody (Cell Signaling, 12173) was used in western blot on human samples at 1:1000 (fig 2). Neoplasia (2016) ncbi
domestic rabbit polyclonal
  • immunocytochemistry; human; 1:200; fig 1
  • western blot; human; 1:200; fig 1
In order to determine the necessity for differentiation-dependent viral life cycle by post-transciptional regulation of KLF4 by high-risk human papillomaviruses, Cell Signaling Technology KLF4 antibody (Cell Signaling Technology, 4038S) was used in immunocytochemistry on human samples at 1:200 (fig 1) and in western blot on human samples at 1:200 (fig 1). PLoS Pathog (2016) ncbi
domestic rabbit polyclonal
  • western blot; human; fig s1
Cell Signaling Technology KLF4 antibody (Cell Signaling Tech, 4038) was used in western blot on human samples (fig s1). Sci Rep (2016) ncbi
domestic rabbit polyclonal
  • immunohistochemistry - paraffin section; human; 1:50; fig 4
In order to characterize the epithelial gain of MEKK3-KLF2/4 signaling due to cerebral cavernous malformations, Cell Signaling Technology KLF4 antibody (Cell signaling, 4038S) was used in immunohistochemistry - paraffin section on human samples at 1:50 (fig 4). Nature (2016) ncbi
domestic rabbit polyclonal
  • western blot; domestic rabbit; 1:500; fig 5a
Cell Signaling Technology KLF4 antibody (Cell Signaling, 4038S) was used in western blot on domestic rabbit samples at 1:500 (fig 5a). J Transl Med (2016) ncbi
domestic rabbit monoclonal (D1F2)
  • western blot; zebrafish ; 1:1000; fig 4
In order to determine factors that regulate mTOR activity during myelination, Cell Signaling Technology KLF4 antibody (Cell Signaling Technology, 1217) was used in western blot on zebrafish samples at 1:1000 (fig 4). J Neurosci (2015) ncbi
domestic rabbit monoclonal (D1F2)
  • immunocytochemistry; human; fig 2
  • western blot; human; fig 2
Cell Signaling Technology KLF4 antibody (Cell Signaling, 12173) was used in immunocytochemistry on human samples (fig 2) and in western blot on human samples (fig 2). Oncotarget (2015) ncbi
MilliporeSigma
domestic rabbit polyclonal
  • western blot; human; 1:1000; loading ...; fig 6d
  • immunohistochemistry - paraffin section; mouse; 1:25; loading ...; fig 5i
  • western blot; mouse; 1:1000; loading ...; fig 6c
MilliporeSigma KLF4 antibody (Sigma, HPA002926) was used in western blot on human samples at 1:1000 (fig 6d), in immunohistochemistry - paraffin section on mouse samples at 1:25 (fig 5i) and in western blot on mouse samples at 1:1000 (fig 6c). Development (2017) ncbi
domestic rabbit polyclonal
  • immunohistochemistry; human; 1:50; loading ...; fig s6d
MilliporeSigma KLF4 antibody (Sigma, HPA002926) was used in immunohistochemistry on human samples at 1:50 (fig s6d). Development (2016) ncbi
domestic rabbit polyclonal
MilliporeSigma KLF4 antibody (Sigma-Aldrich, HPA002926) was used . PLoS Pathog (2015) ncbi
Articles Reviewed
  1. Kaplun D, Starshin A, Sharko F, Gainova K, Filonova G, Zhigalova N, et al. Kaiso Regulates DNA Methylation Homeostasis. Int J Mol Sci. 2021;22: pubmed publisher
  2. Liu J, Cao F, Li X, Zhang L, Liu Z, Li X, et al. ITIH5, a p53-responsive gene, inhibits the growth and metastasis of melanoma cells by downregulating the transcriptional activity of KLF4. Cell Death Dis. 2021;12:438 pubmed publisher
  3. Shen K, Li R, Zhang X, Qu G, Li R, Wang Y, et al. Acetyl oxygen benzoate engeletin ester promotes KLF4 degradation leading to the attenuation of pulmonary fibrosis via inhibiting TGFβ1-smad/p38MAPK-lnc865/lnc556-miR-29b-2-5p-STAT3 signal pathway. Aging (Albany NY). 2021;13:13807-13821 pubmed publisher
  4. Wang P, Zhao L, Gong S, Xiong S, Wang J, Zou D, et al. HIF1α/HIF2α-Sox2/Klf4 promotes the malignant progression of glioblastoma via the EGFR-PI3K/AKT signalling pathway with positive feedback under hypoxia. Cell Death Dis. 2021;12:312 pubmed publisher
  5. Yu S, Guo J, Sun Z, Lin C, Tao H, Zhang Q, et al. BMP2-dependent gene regulatory network analysis reveals Klf4 as a novel transcription factor of osteoblast differentiation. Cell Death Dis. 2021;12:197 pubmed publisher
  6. Sünderhauf A, Hicken M, Schlichting H, Skibbe K, Ragab M, Raschdorf A, et al. Loss of Mucosal p32/gC1qR/HABP1 Triggers Energy Deficiency and Impairs Goblet Cell Differentiation in Ulcerative Colitis. Cell Mol Gastroenterol Hepatol. 2021;12:229-250 pubmed publisher
  7. Deng X, He Y, Miao X, Yu B. ATF4-mediated histone deacetylase HDAC1 promotes the progression of acute pancreatitis. Cell Death Dis. 2021;12:5 pubmed publisher
  8. He L, Bhat K, Duhacheck Muggy S, Ioannidis A, Zhang L, Nguyen N, et al. Tumor necrosis factor receptor signaling modulates carcinogenesis in a mouse model of breast cancer. Neoplasia. 2021;23:197-209 pubmed publisher
  9. Grande G, Milardi D, Martini M, Cenci T, Gulino G, Mancini F, et al. Protein Expression of PTTG-1, OCT-4, and KLF-4 in Seminoma: A Pilot Study. Front Endocrinol (Lausanne). 2019;10:619 pubmed publisher
  10. Sonego M, Pellarin I, Costa A, Vinciguerra G, Coan M, Kraut A, et al. USP1 links platinum resistance to cancer cell dissemination by regulating Snail stability. Sci Adv. 2019;5:eaav3235 pubmed publisher
  11. Senft A, Costello I, King H, Mould A, Bikoff E, Robertson E. Combinatorial Smad2/3 Activities Downstream of Nodal Signaling Maintain Embryonic/Extra-Embryonic Cell Identities during Lineage Priming. Cell Rep. 2018;24:1977-1985.e7 pubmed publisher
  12. Feng J, Jing J, Li J, Zhao H, Punj V, Zhang T, et al. BMP signaling orchestrates a transcriptional network to control the fate of mesenchymal stem cells in mice. Development. 2017;144:2560-2569 pubmed publisher
  13. Periyasamy P, Liao K, Kook Y, Niu F, Callen S, Guo M, et al. Cocaine-Mediated Downregulation of miR-124 Activates Microglia by Targeting KLF4 and TLR4 Signaling. Mol Neurobiol. 2018;55:3196-3210 pubmed publisher
  14. Genovese N, Domeier T, Telugu B, Roberts R. Enhanced Development of Skeletal Myotubes from Porcine Induced Pluripotent Stem Cells. Sci Rep. 2017;7:41833 pubmed publisher
  15. Prieto P, Fernandez Velasco M, Fernández Santos M, Sanchez P, Terrón V, Martín Sanz P, et al. Cell Expansion-Dependent Inflammatory and Metabolic Profile of Human Bone Marrow Mesenchymal Stem Cells. Front Physiol. 2016;7:548 pubmed
  16. Zhao J, Chen C, Guo M, Tao Y, Cui P, Zhou Y, et al. MicroRNA-7 Deficiency Ameliorates the Pathologies of Acute Lung Injury through Elevating KLF4. Front Immunol. 2016;7:389 pubmed
  17. Zimmerlin L, Park T, Huo J, Verma K, Pather S, Talbot C, et al. Tankyrase inhibition promotes a stable human naïve pluripotent state with improved functionality. Development. 2016;143:4368-4380 pubmed
  18. Lee E, Wang J, Yumoto K, Jung Y, Cackowski F, Decker A, et al. DNMT1 Regulates Epithelial-Mesenchymal Transition and Cancer Stem Cells, Which Promotes Prostate Cancer Metastasis. Neoplasia. 2016;18:553-66 pubmed publisher
  19. Gunasekharan V, Li Y, Andrade J, Laimins L. Post-Transcriptional Regulation of KLF4 by High-Risk Human Papillomaviruses Is Necessary for the Differentiation-Dependent Viral Life Cycle. PLoS Pathog. 2016;12:e1005747 pubmed publisher
  20. Tsai S, Huang P, Hsu Y, Peng Y, Lee C, Wang J, et al. Inhibition of hypoxia inducible factor-1α attenuates abdominal aortic aneurysm progression through the down-regulation of matrix metalloproteinases. Sci Rep. 2016;6:28612 pubmed publisher
  21. Cherepanova O, Gomez D, Shankman L, Swiatlowska P, Williams J, Sarmento O, et al. Activation of the pluripotency factor OCT4 in smooth muscle cells is atheroprotective. Nat Med. 2016;22:657-65 pubmed publisher
  22. Zhou Z, Tang A, Wong W, Bamezai S, Goddard L, Shenkar R, et al. Cerebral cavernous malformations arise from endothelial gain of MEKK3-KLF2/4 signalling. Nature. 2016;532:122-6 pubmed publisher
  23. Gehlot P, Shukla V, Gupta S, Makidon P. Detection of ALDH1 activity in rabbit hepatic VX2 tumors and isolation of ALDH1 positive cancer stem cells. J Transl Med. 2016;14:49 pubmed publisher
  24. Kearns C, Ravanelli A, Cooper K, Appel B. Fbxw7 Limits Myelination by Inhibiting mTOR Signaling. J Neurosci. 2015;35:14861-71 pubmed publisher
  25. Nawandar D, Wang A, Makielski K, Lee D, Ma S, Barlow E, et al. Differentiation-Dependent KLF4 Expression Promotes Lytic Epstein-Barr Virus Infection in Epithelial Cells. PLoS Pathog. 2015;11:e1005195 pubmed publisher
  26. Riz I, Hawley T, Hawley R. KLF4-SQSTM1/p62-associated prosurvival autophagy contributes to carfilzomib resistance in multiple myeloma models. Oncotarget. 2015;6:14814-31 pubmed
  27. Shankman L, Gomez D, Cherepanova O, Salmon M, Alencar G, Haskins R, et al. KLF4-dependent phenotypic modulation of smooth muscle cells has a key role in atherosclerotic plaque pathogenesis. Nat Med. 2015;21:628-37 pubmed publisher
  28. Liang W, Wang Y, Liang P, Pan X, Fu W, Yeung V, et al. MiR-25 suppresses 3T3-L1 adipogenesis by directly targeting KLF4 and C/EBPα. J Cell Biochem. 2015;116:2658-66 pubmed publisher
  29. Su C, Sun F, Cunningham R, Rybalchenko N, Singh M. ERK5/KLF4 signaling as a common mediator of the neuroprotective effects of both nerve growth factor and hydrogen peroxide preconditioning. Age (Dordr). 2014;36:9685 pubmed publisher