细胞库
Designations: HmrSv5
Biosafety
Level:
1
Medium
&
Serum:
See Propagation
Organism:
Homo sapiens (human)
Source:
Tissue: mesothelium
Disease: normal
Growth
Properties:
adherent
Morphology:
Epithelial-like
Propagation: The base medium for this cell line is formulated Dulbecco's Modified Eagle's Medium,To
make the complete growth medium, add the following components to the base medium:
4.5g/L glucose ;fetal bovine serum to a final concentration of 10%
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37.0°C
Subculturing: Protocol:
1. Remove and discard culture medium.
2. add fresh 0.25% trypsin, 0.03% EDTA solution, rinse and remove. .
3. Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an
inverted microscope until cell layer is dispersed (usually within 1 to 5 minutes).
4. Add fresh trypsin solution, (1 to 2 ml) and allow the cells to sit at room temperature
(or at 37C) until the cells detach.
5. Add fresh medium, aspirate and dispense into new flasks.
6. Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:5 is recommended
Medium Renewal: Every 2 to 4 days
Preservation: Freeze medium: Complete growth medium 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor phase