This is a Validated Antibody Database (VAD) review about human KL, based on 5 published articles (read how Labome selects the articles), using KL antibody in all methods. It is aimed to help Labome visitors find the most suited KL antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
KL synonym: klotho

Knockout validation
TRANS GENIC
rat monoclonal (KM2076)
  • western blot knockout validation; mouse; 1:500; fig 4
  • immunohistochemistry; mouse; 1:100; fig 5
TRANS GENIC KL antibody (TransGenic, KO603) was used in western blot knockout validation on mouse samples at 1:500 (fig 4) and in immunohistochemistry on mouse samples at 1:100 (fig 5). Nat Commun (2015) ncbi
TRANS GENIC
rat monoclonal (KM2076)
  • western blot knockout validation; mouse; fig 1
  • immunohistochemistry - paraffin section; mouse
  • immunocytochemistry; mouse
In order to report the kidney is the principal contributor of circulating Klotho and regulates anti-aging traits, TRANS GENIC KL antibody (TransGenic, KM2076) was used in western blot knockout validation on mouse samples (fig 1), in immunohistochemistry - paraffin section on mouse samples and in immunocytochemistry on mouse samples . J Am Soc Nephrol (2014) ncbi
Abcam
rabbit monoclonal (EPR6856)
  • immunohistochemistry - paraffin section; human; 1:20; loading ...; fig 5a
  • immunocytochemistry; human; 1:20; fig 6a
  • western blot; human; 1:500; fig 6c
Abcam KL antibody (Abcam, ab181373) was used in immunohistochemistry - paraffin section on human samples at 1:20 (fig 5a), in immunocytochemistry on human samples at 1:20 (fig 6a) and in western blot on human samples at 1:500 (fig 6c). Arthritis Res Ther (2017) ncbi
Invitrogen
rabbit polyclonal
  • immunocytochemistry; human; loading ...; fig 4b
  • western blot; human; loading ...; fig 4c
In order to show that ROS-induced DNA damage reduces metabolic activity, inhibits proliferation, and induces cell cycle arrest followed by activation of p16-, p21-, and p27- mediated DNA damage response pathways, premature senescence, and apoptosis induction, Invitrogen KL antibody (Thermo Scientific, PA5-21078) was used in immunocytochemistry on human samples (fig 4b) and in western blot on human samples (fig 4c). Exp Cell Res (2017) ncbi
TRANS GENIC
rat monoclonal (KM2076)
  • western blot; rat; 1:1000; loading ...; fig 4f
In order to study the neuroprotective effect of latanoprost, TRANS GENIC KL antibody (TransGenic, KO603) was used in western blot on rat samples at 1:1000 (fig 4f). J Neurochem (2017) ncbi
rat monoclonal (KM2076)
  • western blot knockout validation; mouse; 1:500; fig 4
  • immunohistochemistry; mouse; 1:100; fig 5
TRANS GENIC KL antibody (TransGenic, KO603) was used in western blot knockout validation on mouse samples at 1:500 (fig 4) and in immunohistochemistry on mouse samples at 1:100 (fig 5). Nat Commun (2015) ncbi
rat monoclonal (KM2076)
  • western blot knockout validation; mouse; fig 1
  • immunohistochemistry - paraffin section; mouse
  • immunocytochemistry; mouse
In order to report the kidney is the principal contributor of circulating Klotho and regulates anti-aging traits, TRANS GENIC KL antibody (TransGenic, KM2076) was used in western blot knockout validation on mouse samples (fig 1), in immunohistochemistry - paraffin section on mouse samples and in immunocytochemistry on mouse samples . J Am Soc Nephrol (2014) ncbi
Articles Reviewed
  1. Mazzotta C, Manetti M, Rosa I, Romano E, Blagojevic J, Bellando Randone S, et al. Proangiogenic effects of soluble ?-Klotho on systemic sclerosis dermal microvascular endothelial cells. Arthritis Res Ther. 2017;19:27 pubmed publisher
  2. Mytych J, Wos I, Solek P, Koziorowski M. Protective role of klotho protein on epithelial cells upon co-culture with activated or senescent monocytes. Exp Cell Res. 2017;350:358-367 pubmed publisher
  3. Yamamoto K, Sato K, Yukita M, Yasuda M, Omodaka K, Ryu M, et al. The neuroprotective effect of latanoprost acts via klotho-mediated suppression of calpain activation after optic nerve transection. J Neurochem. 2017;140:495-508 pubmed publisher
  4. Sato S, Kawamata Y, Takahashi A, Imai Y, Hanyu A, Okuma A, et al. Ablation of the p16(INK4a) tumour suppressor reverses ageing phenotypes of klotho mice. Nat Commun. 2015;6:7035 pubmed publisher
  5. Lindberg K, Amin R, Moe O, Hu M, Erben R, Östman Wernerson A, et al. The kidney is the principal organ mediating klotho effects. J Am Soc Nephrol. 2014;25:2169-75 pubmed publisher