This IgG fraction antibody was prepared from rabbit antiserum after repeated immunizations with mature length recombinant human MCP-1 protein produced in E.coli.
亚型:
IgG
形态:
Liquid
保存条件:
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
克隆性:
Polyclonal
标记物:
Unconjugated
适应物种:
Human
保质期:
12 months from the shipping date of the product.
抗原来源:
Human
目录编号:
GTX48661
级别:
Primary Antibodies
库存:
Available
供应商:
GeneTex
宿主:
Rabbit
应用范围:
WB, ELISA
浓度:
1 mg/ml (Please refer to the vial label for the specific concentration.)
靶点:
MCP1 / CCL2
抗体英文名:
MCP1 / CCL2 antibody
抗体名:
MCP1 / CCL2 抗体
规格:
50 μg
Western Blot of Anti-human MCP-1 antibody shows detection of recombinant human MCP-1 raised in E.coli. Recombinant (0.1 μg, 8.6 kDa) protein was loaded onto and resolved by SDS-PAGE, then transferred to nitrocellulose. The membrane was blocked with 1% BSA in TBST for 30 min at RT, followed by incubation with GeneTex's Anti-Human MCP-1 antibody. After washing, membrane was probed with secondary antibody Dylight 649 Conjugated Anti-Rabbit IgG (H&L) (Goat) Antibody diluted 1:20,000 in blocking buffer) for 30 min. at RT. Data was collected using Bio-Rad VersaDocR 4000 MP imaging system.