pKOR1是大肠杆菌-金黄色葡萄球菌穿梭载体,主要用于金葡菌的基因敲除。
pKOR1金黄色葡萄球菌基因敲除载体,在大肠杆菌中的抗性为Amp,在金葡菌中筛选抗性为氯霉素(10ug/ml).
生物风对金黄色葡萄球菌的基因敲除具有丰富的实验经验,并且成功敲除过很多金黄色葡萄球菌基因,请有需要对金黄色葡萄球菌进行基因操作技术服务的老师和我们联系。
LOCUS pKOR1 10030 bp DNA circular 2-OCT-2015SOURCE ORGANISM COMMENT This file is created by Vector NTI http://www.biofeng.com/COMMENT VNTDATE|705424862|COMMENT VNTDBDATE|746737717|COMMENT LSOWNER|COMMENT VNTNAME|pKOR1|COMMENT VNTAUTHORNAME|biofeng.com|FEATURES Location/Qualifiers primer 9998..10015 /vntifkey="27" /label=MM13\Forword misc_feature 4824..4840 /vntifkey="21" /label=M13-\R\primer misc_feature complement(7345..7361) /vntifkey="21" /label=M13-R\Primer rep_origin complement(7783..8411) /vntifkey="33" /label=ColE1\origin CDS complement(9859..9927) /vntifkey="4" /label=LacZ\alpha\Reporter misc_binding complement(7367..7389) /vntifkey="20" /label=LacO CDS 8563..9222 /vntifkey="4" /label=Amp\R CDS 1033..1368 /vntifkey="4" /label=Rep(+) CDS complement(1966..2592) /vntifkey="4" /label=Tet\Resistance misc_feature complement(2722..3294) /vntifkey="21" /label=SecY-F misc_feature 5362..6045 /vntifkey="21" /label=Cm\Resistance misc_feature 4883..5114 /vntifkey="21" /label=attP2 CDS 6365..6670 /vntifkey="4" /label=ccdB misc_feature complement(7066..7297) /vntifkey="21" /label=attP2 misc_feature 4883..5114 /vntifkey="21" /label=attP1 misc_feature complement(7066..7297) /vntifkey="21" /label=attP1BASE COUNT 3069 a 1935 c 2129 g 2897 t ORIGIN 1 ctgcagaacg gattgttgat gattacgaaa atattaagag cacagactat tacacagaaa 61 atcaagaatt aaaaaaacgt agagagagtt tgaaagaagt agtgaataca tggaaagagg 121 ggtatcacga aaaaagtaaa gaggttaata aattaaagcg agagaatgat agtttgaatg 181 agcagttgaa tgtatcagag aaatttcaag ctagtacagt gactttatat cgtgctgcga 241 gggcgaattt ccctgggttt gagaaagggt ttaataggct taaagagaaa ttctttaatg 301 attccaaatt tgagcgtgtg ggacagttta tggatgttgt acaggataat gtccagaagg 361 tcgatagaaa gcgtgagaaa cagcgtacag acgatttaga gatgtagagg tacttttatg 421 ccgagaaaac tttttgcgtg tgacagtcct taaaatatac ttagagcgta agcgaaagta 481 gtagcgacag ctattaactt tcggttgcaa agctctagga tttttaatgg acgcagcgca 541 tcacacgcaa aaaggaaatt ggaataaatg cgaaatttga gatgttaatt aaagaccttt 601 ttgaggtctt tttttcttag atttttgggg ttatttaggg gagaaaacat aggggggtac 661 tacgacctcc cccctaggtg tccattgtcc attgtccaaa caaataaata aatattgggt 721 ttttaatgtt aaaaggttgt tttttatgtt aaagtgaaaa aaacagatgt tgggaggtac 781 agtgatagtt gtagatagaa aagaagagaa aaaagttgct gttactttaa gacttacaac 841 agaagaaaat gagatattaa atagaatcaa agaaaaatat aatattagca aatcagatgc 901 aaccggtatt ctaataaaaa aatatgcaaa ggaggaatac ggtgcatttt aaacaaaaaa 961 agatagacag cactggcatg ctgcctatct atgactaaat tttgttaaat gtattagcac 1021 cgttattata tcatgagcga aaatgtaata aaagaaactg aaaacaagaa aaattcaaga 1081 ggacgtaatt ggacatttgt tttatatcca gaatcagcaa aagccgagtg gttagagtat 1141 ttaaaagagt tacacattca atttgtagtg tctccattac atgataggga tactgataca 1201 gaagatagga tgaaaaaaga gcattatcat attctagtga tgtatgaggg taataaatct 1261 tatgaacaga taaaaataat taacagaaga attgaatgcg actattccgc agattgcagg 1321 aagtgtgaaa ggtcttgtga gatatatgct tcacatggac gatcctaata aatttaaata 1381 tcaaaaagaa gatatgatag tttatggcgg tgtagatgtt gatgaattat taaagaaaac 1441 aacaacagat agatataaat taattaaaga aatgattgag tttattgatg aacaaggaat 1501 cgtagaattt aagagtttaa tggattatgc aatgaagttt aaatttgatg attggttccc 1561 gcttttatgt gataactcgg cgtatgttat tcaagaatat ataaaatcaa atcggtataa 1621 atctgaccga tagattttga atttaagagt gtcacaagac actctttttt cgcaccagcg 1681 aaaactggtt taagccgact gcgcaaaaga cataatcgat tcacaaaaaa taggcacacg 1741 aaaaacaagt taagggatgc agtttatgca tcccttaact tacttattaa ataatttata 1801 gctattgaaa agagataaga attgttcaaa gctaatattg tttaaatcgt caattcctgc 1861 atgttttaag gaattgttaa attgattttt tgtaaatatt ttcttgtatt ctttgttatc 1921 ttggttaccg tgaagttacc atcacggaaa aaggttatgc tgcttttaag acccactttc 1981 acatttaagt tgtttttcta atccgcatat gatcaattca aggccgaata agaaggctgg 2041 ctctgcacct tggtgatcaa ataattcgat agcttgtcgt aataatggcg gcatactatc 2101 agtagtaggt gtttcccttt cttctttagc gacttgatgc tcttgatctt ccaatacgca 2161 acctaaagta aaatgcccca cagcgctgag tgcatataat gcattctcta gtgaaaaacc 2221 ttgttggcat aaaaaggcta attgattttc gagagtttca tactgttttt ctgtaggccg 2281 tgtacctaaa tgtacttttg ctccatcgcg atgacttagt aaagcacatc taaaactttt 2341 agcgttatta cgtaaaaaat cttgccagct ttccccttct aaagggcaaa agtgagtatg 2401 gtgcctatct aacatctcaa tggctaaggc gtcgagcaaa gcccgcttat tttttacatg 2461 ccaatacaat gtaggctgct ctacacctag cttctgggcg agtttacggg ttgttaaacc 2521 ttcgattccg acctcattaa gcagctctaa tgcgctgtta atcactttac ttttatctaa 2581 tctagacatc attaattcct cctttttgtt gacactctat cattgataga gttatttgtc 2641 aaactagttt tttatttgga tcccctcgag ttcatgaaaa actaaaaaaa atattgacac 2701 tctatcattg atagagtata attaaaataa gcttgatggg aatatgataa tagaaatacc 2761 attaccaaca ccgaactgag tgatttgatc accaagccat attaagaaag cagttcctgc 2821 tgtcaaaact agtgctatta ataaataact cataattgac tgattgataa tcagcgcacc 2881 tttgagataa ttattaaatt ggaatgccat acctatagat tggataaatg ctaaagaaat 2941 tgctaaataa cgagtaacgt tatttaactt tcttctacct acttcacctt gttttgccca 3001 ttctgagaat ttagggacaa tatccatttg taataattgc attacgattg atgcagtgat 3061 gtagggtaca atacccattg caaaaataga aaatcgtttc aaggctccgc caccaaaagt 3121 atttaataac tcagtggcac cttgagaacc ttggggatta tcaaaagctg caggatttac 3181 tcctggagct ggtatataag tccctatttt aaaaattact aacattgcta gtgtgaagaa 3241 aatcttgtta cgaacctctt ttgttctaaa gaagttcaca agggtttgaa tcattagatc 3301 accccggggt gatctaatga ttcatatcta catcaaatgc tggggcatat gttttaccct 3361 caggtacagg taagtcaccc gcttcaaacg tcttcttaat atgcgtaatt gcatctactc 3421 taaatccatc aatgccttta tcaaaccacc agttcatcat ttcaaataca gcatctctaa 3481 cttccggatt accccaattc aaatcaggtt gttttttact gaataaatgg aaataatatt 3541 gctcagtatt agcatcatat tcccgcgcgc caattgagct cccctttcta tgtatgtttt 3601 ttactagtca tttaaaacga tacattaata ggtacgaaaa agcaactttt tttgcgctta 3661 aaaccagtca taccaataac ttaagggtaa ctagcctcgc cggcaatagt tacccttatt 3721 atcaagataa gaaagaaaag gatttttcgc tacgctcaaa tcctttaaaa aaacacaaaa 3781 gaccacattt tttaatgtgg tcttttattc ttcaactaaa gcacccatta gttcaacaaa 3841 cgaaaattgg ataaagtggg atatttttaa aatatatatt tatgttacag taatattgac 3901 ttttaaaaaa ggattgattc taatgaagaa agcagacaag taagcctcct aaattcactt 3961 tagataaaaa tttaggaggc atatcaaatg aaatttaata aaattgattt agacaattgg 4021 aagagaaaag agatatttaa tcattatttg aaccaacaaa cgacttttag tataaccaca 4081 gaaattgata ttagtgtttt ataccgaaac ataaaacaag aaggatataa attttaccct 4141 gcatttattt tcttagtgac aagggtgata aactcaaata cagcttttag aactggttac 4201 aatagcgacg gagagttagg ttattgggat aagttagagc cactttatac aatttttgat 4261 ggtgtatcta aaacattctc tggtatttgg actcctgtaa agaatgactt caaagagttt 4321 tatgatttat acctttctga tgtagagaaa tataatggtt cggggaaatt gtttcccaaa 4381 acacctatac ctgaaaatgc tttttctctt tctattattc catggacttc atttactggg 4441 tttaacttaa atatcaataa taatagtaat taccttctac ccattattac agcaggaaaa 4501 ttcattaata aaggtaattc aatatattta ccgctatctt tacaggtaca tcattctgtt 4561 tgtgatggtt atcatgcagg attgtttatg aactctattc aggaattgtc agataggcct 4621 aatgactggc ttttataata tgagataatg ccgactgtac tttttacagt cggttttcta 4681 atgtcactaa cctgccccgt tagttgaaga aggtttttat attacagctc cagatccata 4741 tccttctttt tctgaaccga cttctccttt ttcgcttctt tattccaatt gctttattga 4801 cgttgagcct cggaaccggt acccaggaaa cagctatgac catgtaatac gactcactat 4861 acggggatat cagctggatg gcaaataatg attttatttt gactgatagt gacctgttcg 4921 ttgcaacaaa ttgataagca atgctttctt ataatgccaa ctttgtacaa gaaagctgaa 4981 cgagaaacgt aaaatgatat aaatatcaat atattaaatt agattttgca taaaaaacag 5041 actacataat actgtaaaac acaacatatc cagtcactat gaatcaacta cttagatggt 5101 attagtgacc tgtagtcgac taagttggca gcatcacccg acgcactttg cgccgaataa 5161 atacctgtga cggaagatca cttcgcagaa taaataaatc ctggtgtccc tgttgatacc 5221 gggaagccct gggccaactt ttggcgaaaa tgagacgttg atcggcacgt aagaggttcc 5281 aactttcacc ataatgaaat aagatcacta ccgggcgtat tttttgagtt atcgagattt 5341 tcaggagcta aggaagctaa aatggagaaa aaaatcactg gatataccac cgttgatata 5401 tcccaatggc atcgtaaaga acattttgag gcatttcagt cagttgctca atgtacctat 5461 aaccagaccg ttcagctgga tattacggcc tttttaaaga ccgtaaagaa aaataagcac 5521 aagttttatc cggcctttat tcacattctt gcccgcctga tgaatgctca tccggaattc 5581 cgtatggcaa tgaaagacgg tgagctggtg atatgggata gtgttcaccc ttgttacacc 5641 gttttccatg agcaaactga aacgttttca tcgctctgga gtgaatacca cgacgatttc 5701 cggcagtttc tacacatata ttcgcaagat gtggcgtgtt acggtgaaaa cctggcctat 5761 ttccctaaag ggtttattga gaatatgttt ttcgtctcag ccaatccctg ggtgagtttc 5821 accagttttg atttaaacgt ggccaatatg gacaacttct tcgcccccgt tttcaccatg 5881 ggcaaatatt atacgcaagg cgacaaggtg ctgatgacgc tggcgattca ggttcatcat 5941 gccgtttgtg atggcttcca tgtcggcaga atgcttaatg aattacaaca gtactgcgat 6001 gagtggcagg gggggcgtaa acgccgcgtg gatccggctt actaaaagcc agataacagt 6061 atgcgtattt gcgcgctgat ttttgcggta taagaatata tactgatatg tatacccgaa 6121 gtatgtcaaa aagaggtatg ctatgaagca gcgtattaca gtgacagttg acagcgacag 6181 ctatcagttg ctcaaggcat atatgatgtc aatatctccg gtctggtaag cacaaccatg 6241 cagaatgaag cccgtcgtct gcgtgccgaa cgctggaaag cggaaaatca ggaagggatg 6301 gctgaggtcg cccggtttat tgaaatgaac ggctcttttg ctgacgagaa caggggctgg 6361 tgaaatgcag tttaaggttt acacctataa aagagagagc cgttatcgtc tgtttgtgga 6421 tgtacagagt gatattattg acacgcccgg gcgacggatg gtgatccccc tggccagcgc 6481 acgtctgctg tcagataaag tctcccgtga actttacccg gtggtgcata tcggggatga