A compilation of immunohistochemical markers for all major cell types, excluding neural cells.
Adipocytes differentiate from mesenchymal stem cells and compose adipose tissue. Three classes of transcription factor are known to directly influence adipocyte development. These include PPARγ, C/EBPs, and the basic helix–loop–helix family (ADD1/SREBP1c) [1]. There are two types of adipocytes: white, beige and brown adipose tissue. White adipose tissue maintains energy metabolism by storing energy as lipids. Brown adipose tissue (BAT) is a key site of thermogenesis in mammals. Mitochondria in brown adipocytes contain uncoupling protein-1 (UCP1). UCP1-expressing adipocytes, developed in white adipose tissue (WAT), have been named beige adipocytes [2].
Adipose tissue can be stained by perilipin [3]. A number of specific marker genes have been identified for different types of adipocytes. Gene markers such as leptin, HOXC8 and HOXC9 are specific for white adipocytes [4]. Brown adipocytes express Ucp1 [2]. Other important markers of brown adipocytes include CIDEA, and PRDM16 [4], Zic1 [5], Lhx8 [5], Eva1 [6] and Epsti1 [7] and the beige markers include Cd137 [6], Tmem26 [6], Tbx1 [6], Cited1 [7] and Shox2 [8].
Beige adipocytes express a unique gene expression profile. Beige adipocytes express the following markers: Cd137 [6], Tmem26 [6], Tbx1 [6], Cited1 [7] and Shox2 [8], TBX1 and TMEM26 [4]. Beige cell surface proteins CD137 or TMEM26 can be used to identify primary beige fat cell precursors [6].
A recent article reports that amino acid transporter ASC-1, amino acid transporter PAT2, and purinergic receptor P2RX5 are cell surface markers for white, beige, and brown adipocytes, respectively [9]. Flaherty SE et al used ATGL and FABP4 as adipocyte markers [10].
Protein | Top three suppliers | Reference |
---|---|---|
FABP4 / fatty acid binding protein 4 | Cell Signaling Technology 3544 (11), Abcam ab92501 (7), Santa Cruz Biotechnology sc-271529 (7) | [10] |
LEP / leptin | Sino Biological 10221-MM01 (4), Santa Cruz Biotechnology sc-393043 (1) | [4] |
PLIN1 / perilipin | Cell Signaling Technology 9349 (30), Santa Cruz Biotechnology sc-390169 (3), Abcam ab172907 (1) | [3] |
PNPLA2 / ATGL | Cell Signaling Technology 2439 (19), Abcam AB109251 (8), Santa Cruz Biotechnology sc-365278 (5) | [10] |
UCP1 / uncoupling protein 1 | R&D Systems MAB6158 (9), Abcam ab209483 (3), MilliporeSigma SAB1404511 (1) | [2, 3] |
Endothelial cells form the interior surface of all blood vessels, from largest arterias and veins to capillaries. A large number of exclusive and non-exclusive endothelial markers have been identified.
Exclusive endothelial cell markers include von Willebrand factor [11], vascular endothelial cadherin (VE-cadherin, CD144) [12], thrombomodulin (CD141) [13] and Pathologische Anatomie Leiden-endothelium (PAL-E) [14]. Importantly, PAL-E as a specific marker for vascular endothelium, is used to distinguish between vascular and lymphatic endothelium. Other markers distinguishing lymphatic endothelial cells from vascular ones include Lyve-1 [15], Prox1 [15], podoplanin (also a marker for mesothelia [16] ), and vascular endothelial growth factor receptor 3 (VEGFR3) [15, 17, 18]. Double immunostaining for D2-40 podoplanin/CD31 and for PROX1/CD31 was found to distinguishing lymphatic vs. venous blood vessels in dura samples [19]. Besides, MECA-79 and the Daffy antigen receptor for chemokines (DARC) were shown to be highly specific for endothelial cells [11]. H Hu et al stained endothelial cells in human mature arteriovenous fistulae with the Abcam antibody against VWF ( ab11713). Gur-Cohen S et al used endomucin as the marker for blood capillaries [15]. Gifford CA et al labeled endothelial cells in mouse hearts with endomucin [20].
Non-exclusive endothelial specific markers include CLEC4G [21], platelet/EC adhesion molecule-1 (PECAM-1, CD31) [22, 23], transglutaminase 2 / tissue transglutaminase / TG2 [24], vascular endothelial growth factor receptors (VEGFRs): VEGF R1 (Flt-1), VEGF R2 (KDR/Flk-1), and VEGF R3, CD146 (MUC-18, S-endo), UEA-1 (Ulex europaeus I agglutinin), eNOS (endothelial nitric oxide synthase), and Griffonia simplicifolia isolectin B4 (IsoB4). IsoB4 can be delivered in vivo to identify vascular endothelial cells [25, 26].
Other markers, which are important for the activation of endothelial cells, include CD146 (MUC-18, S-endo), thrombomodulin (CD141), ICAM-1 (intercellular adhesion molecule, CD54), E-selectin (CD62E) [27], and apelin [28].
Protein | Top three suppliers | Reference |
---|---|---|
apelin | Santa Cruz Biotechnology sc-293441 (2) | |
CD146 (MUC-18, S-endo) | BD Biosciences 550315 (8), Santa Cruz Biotechnology sc-18837 (5), Abcam ab75769 (5) | |
CLEC4G | [21] | |
Daffy antigen receptor for chemokines (DARC) | Miltenyi Biotec 130-105-685 (1), R&D Systems MAB4139 (1) | |
E-selectin (CD62E) | Invitrogen MA1-22165 (2), BD Biosciences 551145 (2), BioLegend 322606 (1) | |
endomucin | Abcam ab106100 (8) | [15] |
eNOS (endothelial nitric oxide synthase) | BD Biosciences 610297 (22), Cell Signaling Technology 9570 (15), Abcam ab76198 (14) | |
ICAM-1 (intercellular adhesion molecule, CD54) | Santa Cruz Biotechnology sc-8439 (14), Abcam ab2213 (8), Invitrogen 14-0549-82 (8) | |
Lyve-1 (for lymphatic EC) | Abcam ab183501 (1) | [15, 22, 29] |
platelet/EC adhesion molecule-1 (PECAM-1, CD31) | Dako M0823 (115), Invitrogen MA5-13188 (67), Dianova DIA-310 (48) | [21, 29] |
podoplanin (DARC) | Dako M3619 (18), Novus Biologicals NB600-1015 (14), BioLegend 916602 (6) | [23, 29] |
Prox1 (for lymphatic EC) | Angiobio 11-002 (9), Abcam ab199359 (2) | [29] |
TGM2 / TG2 (transglutaminase 2) | Invitrogen MA5-12739 (127), Abcam ab2386 (7), Cell Signaling Technology 3557 (7) | [24] |
thrombomodulin (CD141) | Miltenyi Biotec 130-090-694 (10), BioLegend 344102 (10), Invitrogen MA5-11454 (7) | |
UEA-1 (Ulex europaeus I agglutinin) | BD Biosciences 555693 (5), Santa Cruz Biotechnology sc-51733 (2), BioLegend 311308 (2) | |
vascular endothelial cadherin (VE-cadherin, CD144) | Santa Cruz Biotechnology sc-9989 (36), Cell Signaling Technology 2500 (26), Invitrogen 14-1449-82 (9) | |
VEGF R1 (Flt-1) | Abcam ab32152 (16), R&D Systems FAB4711P (5), Santa Cruz Biotechnology sc-271789 (3) | |
VEGF R2 (KDR/Flk-1) | Cell Signaling Technology 2479 (82), Santa Cruz Biotechnology sc-6251 (30), BD Biosciences 560494 (4) | |
VEGFR3 | R&D Systems FAB3492P (6), BioLegend 356202 (4), Santa Cruz Biotechnology sc-28297 (1) | |
von Willebrand factor | Dako M0616 (17), Invitrogen MA5-14029 (13), Santa Cruz Biotechnology sc-365712 (5) | [30] |
Epithelial cells (EpC) originate from all embryonic germ layers (ectoderm, endoderm and mesoderm). Epidermal cells have ectodermal origin. Predominant epidermal cells are keratinocytes, which differentiate from epidermal stem cells. There are a large number of subtype-specific epithelial markers.
Keratines (K) are keratin-containing proteins detected in the cytoplasm of EpCs [31-33]. There are acidic type I and basic type II Ks. Keratins 1-3 are expressed by squamous EpCs. The expression of K5 and K6 are specific for mesothelial cells (also podoplanin [16] ) and K7 for ductal and glandular EpCs. K8 is expressed in EpCs of gastrointestinal tract (including stomach, colon, small intestine, gall bladder, liver, pancreas) and mammary gland ducts [34]. EpCs of skin, keratinocytes, express the following specific markers: keratins 1, 5, 10, 14, 15 and 16. K10 is expressed in suprabasal layer of squamous epithelia. K18 serves as a marker of proliferating malignant EpCs [35]. K Yoshida et al isolated bronchial epithelial cells through flow cytometry using EpCAM as a marker [36].
Protein | Top three suppliers | Reference |
---|---|---|
DCLK1 (for Tuft cells only) | Santa Cruz Biotechnology sc-271390 (6), Abcam ab109029 (1) | [37, 38] |
E-cadherin | Cell Signaling Technology 3195 (328), BD Biosciences 610181 (311), Invitrogen 13-1700 (278) | [39] |
Epcam | Cell Signaling Technology 2929 (16), BioLegend 324202 (13), Invitrogen MA1-10195 (12) | [40, 41] |
epithelial membrane antigen (EMA, CD227, MUC-1) | Invitrogen MA5-11202 (59), Dako M0613 (33), Novus Biologicals NB120-22711 (12) | |
epithelial sodium channel β | Santa Cruz Biotechnology sc-25354 (4) | |
FcR | BioLegend 303202 (10), BD Biosciences 557333 (8), Bio-Rad MCA1075 (6) | |
ICAM-1 | Santa Cruz Biotechnology sc-8439 (14), Abcam ab2213 (8), Invitrogen 14-0549-82 (8) | |
keratin 1 | Invitrogen MA1-82041 (104), BD Biosciences 349205 (20), Novus Biologicals NB100-2756 (10) | |
keratin 2 | Invitrogen MA1-82041 (104), Progen 61806 (2) | |
keratin 3 | Invitrogen MA1-82041 (104), Abcam ab77869 (5), Santa Cruz Biotechnology sc-80000 (3) | |
keratin 5 | Invitrogen MA1-82041 (104), Dako M7237 (40), MilliporeSigma C2562 (21) | |
keratin 6 | Invitrogen MA1-82041 (104), BD Biosciences 349205 (20), Abcam ab18586 (3) | |
keratin 7 | Invitrogen MA1-82041 (104), Dako M7018 (52), Abcam ab181598 (12) | |
keratin 8 | Invitrogen MA1-82041 (104), Developmental Studies Hybridoma Bank TROMA-I (59), MilliporeSigma C2562 (21) | [35] |
keratin 10 | Invitrogen MA1-82041 (104), MilliporeSigma C2562 (21), Santa Cruz Biotechnology sc-81714 (10) | |
keratin 14 | Invitrogen MA5-11599 (119), Abcam ab7800 (29), Santa Cruz Biotechnology sc-53253 (8) | |
keratin 15 | Invitrogen MA1-82041 (104), Dako M3515 (73), Abcam ab80522 (7) | |
keratin 16 | Invitrogen MA1-82041 (104), MilliporeSigma C2562 (21), Santa Cruz Biotechnology sc-53255 (3) | |
keratin 18 | Invitrogen MA1-82041 (104), Abcam ab668 (21), MilliporeSigma C2562 (21) | [41, 42] |
LFA-1 | Invitrogen MA1-19003 (7), BioLegend 301202 (5), BD Biosciences 555381 (5) | |
LFA-2 | Invitrogen 16-0029-85 (8), BD Biosciences 555324 (5), BioLegend 300202 (3) | |
PSA (prostate specific antigen) | Santa Cruz Biotechnology sc-7316 (10), Cell Signaling Technology 5365 (5), Dako M0750 (3) | |
surfactant protein A | Abcam AB51891 (4) | |
surfactant protein B | Invitrogen MA5-13975 (5), Santa Cruz Biotechnology sc-133143 (2) | |
surfactant protein D | Abcam AB17781 (5), Santa Cruz Biotechnology sc-59695 (2) | |
survivin | Cell Signaling Technology 2808 (54), Santa Cruz Biotechnology sc-17779 (33), Novus Biologicals NB500-238 (28) | |
VLA-1 | BD Biosciences 559594 (7), Invitrogen MA49A0 (2), Bio-Rad MCA1133F (2) | |
VLA-2 | Abcam ab133557 (5), Bio-Rad MCA2025 (4), BD Biosciences 555498 (3) | |
VLA-3 | Santa Cruz Biotechnology sc-374242 (3), BD Biosciences 556025 (3), Abcam ab8988 (2) | |
VLA-4 | BD Biosciences 555502 (11), BioLegend 304302 (7), Invitrogen 12-0499-42 (6) | |
VLA-5 | Abcam ab150361 (10), BD Biosciences 555617 (5), Santa Cruz Biotechnology sc-376199 (4) | |
VLA-6 | BD Biosciences 555734 (26), BioLegend 313602 (20), Abcam ab20142 (7) |
Other markers of EpCs include E-cadherin, epithelial membrane antigen (EMA, CD227, MUC-1) (expressed by most secretory EpCs), epithelial sodium channels α, β, γ, δ, prostate-specific antigen (PSA) (expressed by prostate EpCs), surfactant protein A-D (expressed by pulmonary epithelia, pro-surfactant protein C as a marker for type II alveolar epithelial cells [43] ), survivin (cells of epithelial carcinoma). Also, they express the receptors for the Fc part of the IgG (FcR), integrin molecules: VLA-1, 2, 3, 4, 5, 6, adhesion molecules LFA-1, LFA-2, ICAM-1 [1548035). The cells, forming the lining of the gastrointestinal tract, develop from endoderm. The lining of body cavities develops from mesoderm.
Tuft cells (also called brush cells) are a specialized type of epithelial cells able to use taste receptors and other surface proteins to detect pathogens. They are found in digestive and respiratory tracts. Both structural components (villin, fimbrin, α- or β-tubulin, Ac-tubulin, ankyrin, CK-18, neurofilaments, Dclk1) and taste cell-related proteins (α-gustducin, Trpm5, T1R1/T1R3) can serve as markers, in addition to Ptgs1, Ptgs2, H-Pgds, UEA1 lectin and Sox9 [44]. Wilen CB et al used DCLK1 and CK18 to identify Tuft cells in mouse ileum and colon [42]. Miller CN et al transcriptionally profiled a subset of thymic cells and found them to be similar to intestinal Tuft cells and confirmed the expression of KRT18/8 and DCLK1 among these thymic Tuft cells [37]. Nadjsombati MS et al used DCLK1 as a marker in the mouse small intestinal Tuft cells [38]. Lei W et al used DCLK1 staining as well to identify Tuft cells in mouse jejunum tissues [45].
Dendritic cells (DC) have the key role in adaptive immunity inducing antigen-specific immunity. Regarding both functions and localization, DCs can be classified into three subsets: conventional, plasmacytoid and dermal (skin located) DCs [46].
Conventional DCs reside in lymph nodes, spleen and thymus. In mice, conventional DCs can be divided into CD8+ (with phenotypes CD8+CD205+SIRPa-CD11b- in spleen and CD11chi MHCII+ CD8+ CD205+ in lymph nodes) and CD8- DCs [47]. These cells activate T cells toward Th1 and Th2 differentiation respectively.
Plasmacytoid DCs (pDC) belong to the second DC subset, which reside in lymph nodes, spleen, thymus and bone marrow. Human pDCs mature in the bone marrow and play specific role in anti-viral immunity by secreting anti-viral and pro-inflammatory cytokines including IFNs, TNFα, IL-6 and IL-12. These cells are composed of two subsets: CD2high and CD2low pDCs. Both human and murine pDCs express the following markers: B220/CD45R, CD11c [48], TLR7 and TLR9, IRF7, IRF8 [49] and BDCA2 [50].
The third subset of DCs is located in skin and develops from myeloid lineage. There are two distinct subgroups of the skin DCs: epidermal Langerhans cells (LCs) and dermal DCs. LCs are identified by the presence of Langerin-containing Birbeck granules and expression of the following markers: CD1a, CD45. In addition, among dermal DCs two subpopulations have been identified: CD103+ CD11blow Langerin+ and CD103- CD11bhi Langerin- DCs [51]. Dermal DCs also express CD14. Mature DCs also express CD1a, CD1b and CD1c molecules, which present lipid and glycolipid antigens to CD1/restricted T cells [52].
Several factors regulating differentiation of DCs have been identified. Human CD14+ monocytes differentiate into DCs when cultured with GMC-SF+ IL4 [53]. Also, human CD34+ cells can differentiate into DCs when cultured with GMC-SF + TNF [54]. Mouse bone marrow cells cultured with GMCSF+ TNF+ stem cell factor (SCF] can differentiate into DCs [55].
There are other DC markers which have different degree of specificity. CD83 is a specific marker of mature DCs [56]. CD21 and clusterin are markers of follicular DCs [57, 58]. In addition, DCs express: ADAM19 [59], CD86 [60], DC-LAMP (CD208] [61, 62], DC-SIGN (CD209) [63], DEC-205 [64], CLIP-170/restin [65], NLDC-145 [66]. MADDAM (metalloprotease and disintegrin dendritic antigen marker) is a marker of DC differentiation [67].
Protein | Top three suppliers | Reference |
---|---|---|
B220/CD45R | BioLegend 103202 (149), Invitrogen 14-0452-86 (137), BD Biosciences 560777 (68) | |
CD1a | BioLegend 300102 (12), Invitrogen MA5-12526 (12), BD Biosciences 555805 (11) | |
CD1b | Invitrogen AHS0198 (5), BioLegend 329102 (3), BD Biosciences 555969 (3) | |
CD1c | BioLegend 331501 (20), Miltenyi Biotec 130-090-695 (9), Invitrogen AHS0198 (5) | [41] |
CD11c | BD Biosciences 550375 (39), BioLegend 301601 (17), Abcam ab1211 (15) | [68] |
CD14 | BD Biosciences 555396 (86), Invitrogen MHCD1400 (48), BioLegend 301802 (38) | |
CD21 | Invitrogen MA5-11417 (11), BD Biosciences 555421 (9), Dako M0784 (6) | |
CD45 | BioLegend 103202 (149), Invitrogen 14-0452-86 (137), BD Biosciences 560777 (68) | [68] |
CD83 | BD Biosciences 556854 (20), BioLegend 305302 (13), Invitrogen MHCD8304 (7) | |
CD86 | BioLegend 305402 (36), Invitrogen MA1-10293 (33), BD Biosciences 555656 (23) | |
CD123 / IL3RA | BD Biosciences 554527 (15), Invitrogen 14-1239-82 (11), BioLegend 306027 (11) | [69] |
CD208 / DC-LAMP | Novus Biologicals ddx0191p-100 (2), Dentritics DDX0191 (2), BD Biosciences 558126 (2) | |
CD209 / DC-SIGN | BD Biosciences 551186 (11), Invitrogen 14-2099-80 (5), BioLegend 330112 (2) | |
CD303 / BDCA2 / CLEC4C | Miltenyi Biotec 130-090-690 (18), BioLegend 354215 (5), Invitrogen 25-9818-42 (2) | [69] |
CLIP-170 / restin | Santa Cruz Biotechnology sc-28325 (1) | |
CLU / clusterin | Santa Cruz Biotechnology sc-5289 (7), Sino Biological 11297-R210 (3), Abcam ab92548 (2) | |
CST3 / cystatin C | Abcam ab109508 (5) | [69] |
DEC-205 | Invitrogen MA5-13365 (5), Santa Cruz Biotechnology sc-59158 (1), Bio-Rad MCA1651GA (1) | |
FCER1A | BioLegend 134301 (20), Invitrogen MA1-4997 (6), Abcam ab54411 (2) | [41, 69] |
IL-6 | Abcam ab9324 (16), Invitrogen AHC0562 (11), Santa Cruz Biotechnology sc-28343 (5) | |
IL-12 | Invitrogen AHC9122 (4), Santa Cruz Biotechnology sc-74147 (1), Abcam ab131039 (1) | |
IRF7 | Santa Cruz Biotechnology sc-74472 (1), BioLegend 656008 (1), Cell Signaling Technology 13014 (1) | |
IRF8 | Cell Signaling Technology 5628 (5), Invitrogen 17-9852-82 (2), Santa Cruz Biotechnology sc-365042 (2) | |
TNFα | BD Biosciences 559071 (44), Invitrogen 14-7349-85 (38), BioLegend 502902 (34) | |
TLR7 | Santa Cruz Biotechnology sc-57463 (2), Invitrogen MA5-16249 (2), R&D Systems IC5875P (2) | |
TLR9 | Novus Biologicals NBP2-24729 (24), Invitrogen 14-9099-82 (5), Santa Cruz Biotechnology sc-47723 (4) |
Glial cells are the cells located in the nervous system which provide protection and nutrition for the neurons, regulate migration of neurons in early development, communications between neurons and neurotransmitter release. The glial cell lineage includes microglia and macroglia. Microglia are extensively discussed in Labome's article Macrophage Markers.
Macroglia consists of astrocytes, oligodendrocytes, ependymal cells, radial glia, Schwann cells, satellite cells and enteric glial cells. Astrocytes are classified into type 1 astrocytes (Ran2+, GFAP+, FGFR3+, A2B5-) and type 2 astrocytes (A2B5+, GFAP+, FGFR3-, Ran 2-). There are two main groups of Schwann cells: myelinating (specific markers: proteins S-100, Myelin protein zero (P-Zero) and Myelin basic protein (MBP)) and non-myelinating (specific markers: S-100 and Glial fibrillary acidic protein (GFAP)). Precursors of oligodendrocytes express platelet-derived growth factor (PDGF) receptors, which bind PDGF [70]. Ependymal cells express S-100, vimentin, GFAP, BLBP, 3A7 and 3CB2 [71]. Schwann cells are the main glial cells of the peripheral nervous system. Specific markers for identification of Schwann cells are S-100, myelin basic protein (MBP) and myelin protein zero (MPZ). Satellite cells provide support for neurons in peripheral nervous system and express CD45 and markers of myeloid lineage CD14, CD68, and CD11b [72]. The specific markers for enteric glial cells include: S-100 protein, the neurofilament protein and the protein gene product 9.5 (PGP) [73]. Nott A et al isolated oligodendrocyte nuclei from human brain tissues through FANS with an antibody against OLIG2 and astrocyte nuclei with an antibody against LHX2 [74].
Protein | Top three suppliers | Reference |
---|---|---|
CD45 | BioLegend 103202 (149), Invitrogen 14-0452-86 (137), BD Biosciences 560777 (68) | |
CD68 | Invitrogen MA5-13324 (88), Dako M0876 (88), Abcam ab955 (46) | |
GFAP | MilliporeSigma G3893 (234), Invitrogen 13-0300 (96), Cell Signaling Technology 3670 (66) | |
CD14 | BD Biosciences 555396 (86), Invitrogen MHCD1400 (48), BioLegend 301802 (38) | |
vimentin | Cell Signaling Technology 5741 (244), Invitrogen MA5-11883 (218), Abcam ab92547 (115) | |
CD11b | Invitrogen MA1-10080 (226), BioLegend 101249 (109), Abcam ab133357 (42) | |
proteins S-100 | Invitrogen MA5-12969 (57), Abcam ab4066 (16), Biogenex MU058-5UC (3) | |
MBP / myelin basic protein | Abcam ab7349 (42), BioLegend 808401 (23), Santa Cruz Biotechnology sc-271524 (8) | |
platelet-derived growth factor (PDGF) receptor | Abcam ab32570 (50), Cell Signaling Technology 3169 (44), BD Biosciences 558821 (9) | |
neurofilament protein | Dako M0762 (35), BioLegend 837904 (24), Cell Signaling Technology 2837 (11) | |
MHC class I | BioLegend 311402 (29), Invitrogen MA1-19027 (14), BD Biosciences 555552 (8) | |
protein gene product 9.5 (PGP) | Cell Signaling Technology 13179 (8), Abcam ab8189 (7), Invitrogen 480012 (7) | |
FGFR3 | Santa Cruz Biotechnology sc-13121 (6), Cell Signaling Technology 4574 (5), Abcam ab155960 (2) | |
MHC class II | Santa Cruz Biotechnology sc-65320 (1) | |
Myelin protein zero (P-Zero) | Abcam ab183868 (1) |
Bone marrow contains hematopoietic stem cells (HSC) which give raise to three main classes of blood cells: leukocytes, erythrocytes and thrombocytes. The main phenotype of human HSCs is: CD34+, CD38low/-, CD59+, Thy1+, c-Kit+, Lin-. Mouse HCSs can be identified as: CD34low/-, CD38+, Thy1+/low, SCA-1+, c-Kit+, Lin-. The other markers expressed by HSCs are: CD90, CD93, CD105, CD110, Ly-6A/E (Sca-1), CD111, CD135 (Flk-2), CD150 (SLAM), CD184 (CXCR4), CD202b, CD243 (MDR-1), CD271 (NFGR), CD309 (VEGFR2) and CD338 [75].
The main processes of differentiation in bone marrow include myelopoiesis, erythropoiesis and megakaryocyte lineage development. In the process of myelopoiesis the following cell types are generated: granulocytes, monocytes and mast cells. There are three different types of granulocytes generated in the bone marrow: eosinophils, basophils and neutrophils. Eosinophils differentiate from bone marrow in response to IL-3, IL-5 and GM-CSF [76-78]. Both mouse and human neutrophils express the following markers: Ly-6G [79, 80], CD11b [80], FcεRI, CD123, CD49b / DX-5, CD69, Thy-1.2, 2B4. Specific surface marker for monocytes is CD14 (CD14+ cells). ElTanbouly MA et al obtained mouse neutrophils as CD11b+ Ly6G+ Ly6C−, and monocytes as CD11b+ Ly6C+ Ly6G− [80]. Markers constantly expressed by bone marrow mast cells include: CD9, CD29, CD33, CD43, CD44, CD49d, CD49e, CD51, CD71, CD117, and Fc(epsilon)RI [81].
Protein | Top three suppliers | Reference |
---|---|---|
CD34 | Invitrogen MA1-10202 (162), Abcam ab81289 (43), BD Biosciences 555824 (26) | |
CD14 | BD Biosciences 555396 (86), Invitrogen MHCD1400 (48), BioLegend 301802 (38) | |
Ly-6A/E (Sca-1) | Cell Signaling Technology 9664 (530), BD Biosciences 559565 (43), Novus Biologicals NB100-56708 (42) | |
CD44 | BioLegend 103002 (157), Invitrogen 14-0441-81 (128), BD Biosciences 550392 (34) | |
CD71 | Invitrogen 13-6800 (449), BD Biosciences 555534 (17), BioLegend 334102 (10) | |
CD11b | Invitrogen MA1-10080 (226), BioLegend 101249 (109), Abcam ab133357 (42) | |
c-Kit | Invitrogen 14-1172-85 (19), BioLegend 313201 (19), Cell Signaling Technology 3074 (18) | |
CD117 | Invitrogen 14-1172-85 (19), BioLegend 313201 (19), Cell Signaling Technology 3074 (18) | |
CD29 | BD Biosciences 610467 (19), Abcam ab30394 (18), Cell Signaling Technology 9699 (16) | |
CD184 (CXCR4) | BioLegend 306502 (16), Invitrogen 35-8800 (16), BD Biosciences 555971 (14) | |
CD38 | BioLegend 303502 (34), BD Biosciences 564498 (31), Invitrogen MA1-19316 (27) | |
Thy1 | BD Biosciences 555595 (30), BioLegend 328101 (13), Abcam ab92574 (13) | |
CD90 | BD Biosciences 555595 (30), BioLegend 328101 (13), Abcam ab92574 (13) | |
Thy-1.2 | BD Biosciences 555595 (30), BioLegend 328101 (13), Abcam ab92574 (13) | |
CD309 (VEGFR2) | Cell Signaling Technology 2479 (82), Santa Cruz Biotechnology sc-6251 (30), BD Biosciences 560494 (4) | |
CD69 | BioLegend 310902 (34), BD Biosciences 560740 (31), Invitrogen MA1-207 (26) | |
CD271 (NFGR) | Invitrogen MA5-13311 (15), BD Biosciences 557196 (10), Abcam ab52987 (8) | |
CD105 | Invitrogen MHCD10500 (14), BD Biosciences 555690 (10), Abcam ab11414 (8) | |
CD59 | Invitrogen MA1-19133 (14), Hycult Biotech HM2120 (3), Santa Cruz Biotechnology sc-133171 (2) | |
CD123 | BD Biosciences 554527 (15), Invitrogen 14-1239-82 (11), BioLegend 306027 (11) | |
CD33 | BD Biosciences 561157 (17), BioLegend 303419 (12), Beckman Coulter A54824 (7) | |
CD43 | BD Biosciences 555474 (10), Invitrogen MA5-16646 (2), Santa Cruz Biotechnology sc-51727 (2) | |
CD49e | Abcam ab150361 (10), BD Biosciences 555617 (5), Santa Cruz Biotechnology sc-376199 (4) | |
CD202b | Cell Signaling Technology 4224 (9), Abcam ab24859 (4), R&D Systems MAB313 (2) | |
IL-5 | BioLegend 504302 (14), BD Biosciences 554391 (10), Invitrogen MA5-23690 (6) | |
CD243 (MDR-1) | Santa Cruz Biotechnology sc-55510 (16), Abcam ab170904 (14), Invitrogen MA1-26528 (14) | |
CD338 | Abcam ab3380 (17), Enzo Life Sciences ALX-801-036-C250 (10), Santa Cruz Biotechnology sc-58222 (4) | |
FcεRI | BioLegend 134301 (20), Invitrogen MA1-4997 (6), Abcam ab54411 (2) | |
CD49b (DX-5) | Abcam ab133557 (5), Bio-Rad MCA2025 (4), BD Biosciences 555498 (3) | |
CD51 | Abcam ab179475 (12), BD Biosciences 611012 (5), Santa Cruz Biotechnology sc-376156 (4) | |
GM-CSF | Invitrogen 14-1169-82 (2), BioLegend 305906 (2), Santa Cruz Biotechnology sc-21764 (1) | |
CD9 | Abcam ab92726 (36), Santa Cruz Biotechnology sc-13118 (15), Invitrogen AHS0902 (14) | |
CD150 (SLAM) | BioLegend 306302 (7), BD Biosciences 559592 (2), Abcam ab2604 (1) | |
CD49d | BD Biosciences 555502 (11), BioLegend 304302 (7), Invitrogen 12-0499-42 (6) | |
Lin | Cell Signaling Technology 3695 (3), Santa Cruz Biotechnology sc-293120 (1), Invitrogen MA1-016 (1) | |
CD93 | BD Biosciences 551531 (2) | |
CD110 | BD Biosciences 562159 (2) | |
IL-3 | Invitrogen AHC0832 (1) | |
2B4 | BioLegend 329502 (11), Santa Cruz Biotechnology sc-136172 (6), Beckman Coulter IM1607 (4) | |
CD111 | Santa Cruz Biotechnology sc-21722 (5), Invitrogen 37-5900 (5), BioLegend 340404 (2) | |
CD135 (Flk-2) | BD Biosciences 558996 (5), BioLegend 313302 (4), Cell Signaling Technology 3464 (2) |
Natural Killer (NK) cells play the important role in immune response against malignant and infected cells. During NK lineage development, human NK cells pass through five main stages of differentiation. During these five stages NK cells express distinct sets of markers: 1) CD34+ CD45RA+ CD117− CD161− CD94−; 2) CD34+ CD45RA+ CD117+ CD161+/− CD94−; 3) CD34− CD117+ CD161+ NKp46− CD94−; 4) CD34− CD117+/− NKp46+ CD94+ CD16− CD56bright; 5) CD34− CD117− NKp46+ CD94+/− CD16+ CD56dim [82]. There are two major populations of human blood NK cells which are defined on the basis of the surface expression intensity of CD56 [83, 84] and the low-affinity Fc receptor CD16 [85]. A larger population of CD56dim NK cells (∼90%) expresses high levels of CD16, whereas a minor subset of CD56bright NK cells expresses limited CD16.
In contrast to human NK cells, murine NK cellular subsets can be distinguished from each other by the expression of CD27 and CD11b markers. These subsets include immature CD11b- NK cells, CD27+ NK cells and mature (terminal) CD27-CD11b+ NK cells. NK cells can be activated by several interleukins: IL-12, IL-2, IL-15, IL-18. Also, NK cells express receptors for CXC, CC and C chemokines, which are important for the regulation of NK functions [86]. In addition, NK cells express receptors recognizing MHC class I molecules (human KIRs, the rodent Ly49 and CD94/NKG2), NKp46, FcgRII and non-MHC-binding NK receptors (NKR-P1 (CD161)) [87], natural cytotoxicity receptors (NCR) and 2B4) [88], NKG2A and NKp80 [89], CD107a - a functional marker NK cell activity [90], CD69 - NK cell activation marker [87], CD335/NKp46 [91], BAT [92], CD57/HNK1 [93], NKH1 (N901) [94], DPIV (dipeptidyl peptidase IV) - a surface marker of NK cells [95], H25 [96].
Protein | Top three suppliers | Reference |
---|---|---|
2B4 | BioLegend 329502 (11), Santa Cruz Biotechnology sc-136172 (6), Beckman Coulter IM1607 (4) | |
CD11b | Invitrogen MA1-10080 (226), BioLegend 101249 (109), Abcam ab133357 (42) | |
CD16 | BD Biosciences 550383 (85), Invitrogen MA1-10112 (35), BioLegend 302002 (20) | |
CD27 | BioLegend 302839 (36), BD Biosciences 561408 (36), Invitrogen 14-0271-82 (32) | |
CD34 | Invitrogen MA1-10202 (162), Abcam ab81289 (43), BD Biosciences 555824 (26) | |
CD45RA | BioLegend 103202 (149), Invitrogen 14-0452-86 (137), BD Biosciences 560777 (68) | |
CD56 / NCAM1 | BioLegend 318302 (56), BD Biosciences 564488 (54), Invitrogen MA1-06801 (26) | [41, 69] |
CD57 / HNK1 | Invitrogen MA5-12008 (21), BD Biosciences 559048 (7), BioLegend 359602 (6) | |
CD69 | BioLegend 310902 (34), BD Biosciences 560740 (31), Invitrogen MA1-207 (26) | |
CD94 / NKG2 | BD Biosciences 555887 (13), BioLegend 305504 (5), Invitrogen 11-0949-42 (1) | |
CD107a | BD Biosciences 555798 (58), Developmental Studies Hybridoma Bank H4A3 (56), Cell Signaling Technology 9091 (54) | |
CD117 | Invitrogen 14-1172-85 (19), BioLegend 313201 (19), Cell Signaling Technology 3074 (18) | |
CD128 / CD181 / CXCR1 | BD Biosciences 551080 (3), Santa Cruz Biotechnology sc-7303 (2), Invitrogen 14-1819-82 (2) | |
CD161 / KLRB1 / NK1.1 | BioLegend 339902 (18), BD Biosciences 556079 (11), Miltenyi Biotec 130-092-676 (8) | [69, 97] |
CD191 / CCR1 | BioLegend 362903 (1), R&D Systems MAB5986 (1), BD Biosciences 557914 (1) | |
CD335 / NKp46 / LY94 / NCR1 | BioLegend 331902 (14), Beckman Coulter A66902 (7), BD Biosciences 557911 (6) | |
DPIV (dipeptidyl peptidase IV) | BioLegend 302702 (4), BD Biosciences 555435 (3), Invitrogen MA1-35147 (2) | |
FcgRII | BioLegend 303202 (10), BD Biosciences 557333 (8), Bio-Rad MCA1075 (6) | |
GNLY / granulysin / NKG5 / LAG2 | BioLegend 348008 (6), BD Biosciences 558254 (1) | [69] |
IL-2 | BioLegend 500301 (17), BD Biosciences 554562 (16), Invitrogen 14-7029-81 (9) | |
IL-12 | Invitrogen AHC9122 (4), Santa Cruz Biotechnology sc-74147 (1), Abcam ab131039 (1) | |
IL-15 | Santa Cruz Biotechnology sc-8437 (1) | |
IL-18 | Abcam ab207324 (1), MBL International D044-3 (1) | |
KIR | BioLegend 312712 (8), Miltenyi Biotec 130-104-483 (1), Beckman Coulter A60795 (1) | |
NKG2A | Beckman Coulter IM2750 (33), R&D Systems MAB1059-100 (1), Miltenyi Biotec 130-114-092 (1) | |
NKp80 | BioLegend 346708 (1), Miltenyi Biotec 130-094-843 (1) |
The following discusses markers for less commonly studied cell types.
L Pellegrini et al used TTR / transthyretin, CLIC6 and HTR2C as markers for choroid plexus [98].
Pericentriolar material 1 (PCM1) and cTroponins I and T can be used to label cardiomyocyte nuclei [99, 100]. Connexin 43 (cx43) is also a good marker for cardiomyocytes [101]. Wheat germ agglutinin lectin can label cardiomyocyte fibrosis [99, 102]. MF20 from DSHB, an antibody against sarcomeric isoforms of myosin heavy chain, can stain cardiomyocyte differentiation [103].
Enterocytes, or intestinal absorptive cells, are a type of epithelial cells response for the absorption of water, ions, and other nutrients in the small intestines. AldolaseB is commonly used as their marker [104].
T Yokota et al labeled fibroblasts with vimentin [105]. H Qian et al, on the other hand, used fibronectin as a marker for fibroblasts [106].
Germ cells include specific cell types involved in reproduction. They include gametes (the sperm and eggs) and gonocytes regulating the production of gametes. The specific markers of germ cells include 4C9 [107], GCNA1 (germ cell nuclear antigen 1, GCNA-1) [108, 109], DAZ-like 1(DAZL1) [110], VASA [111], ZAR1 (zygotic arrest 1) [108], TEX101 [112]. In addition, RBM (RNA-binding motif) [113] and tesmin [114].
Granulosa cells form a barrier around ovarian oocyte follicles. As the follicles mature, the granulosa cells multiply to form many layers around the oocyte. Granulosa cells produce estradiol before ovulation and secrete progesterone after ovulation. The main markers of granulose cells are AMH (anti-mullerian hormone) [115], Follicle regulatory protein (FRP) [116], inhibin [117, 118], MCAM (Melanoma cell adhesion molecule, CD146) [119], fibronectin [120].
Hepatocytes are the parenchymal cells of liver. Aizarani N et al used HP and ASGR1 as markers for hepatocytes to build a human liver atlas using single-cell RNA-seq [21].
Jurkat cells are the cells of CD4+ T cell leukemia line. These cells express specific markers of T cells including CD3, CD4, CD45 [121] and produce interleukin-2 (IL-2). In addition, Jurkat cells express chemokine receptors CCR1-10 and CXCR4.
Large luteal cells are located in the corpus luteum and produce progesterone and oxytocin. They are derived from granulose cells. Markers of large luteal cells include CYP11A1 [122], luteinizing hormone receptor [123], phosphorylated Akt [124].
Mast cells are granulated cells of hematopoietic origin found in most tissues. Mast cells contain large amount of granules rich in histamine and heparin and play an important role in allergy and anaphylaxis. Specific markers of mast cells are tryptase [125], high affinity IgE receptor [126], CD25 [126], CD45 [127], CD23 [126], CD117 (c-Kit) [41, 127] and CD203c [126].
Neuroendocrine cells are activated by neurotransmitters and release hormones. Ouadah Y et al used CGRP as a marker for pulmonary neuroendocrine cell marker [128].
Paneth cells are one of the main cell type of the small intestine epithelium. They are part of the immune defence system, since they synthesize and secrete antimicrobial peptides and proteins and lysozyem. Lysozyme is commonly used as their marker [104].
Pericytes, located around the capillaries and venules, help maintain homeostatic and hemostatic functions. F Binet et al labeled mouse retinal vascular pericytes with NG2 [25]. Nortley R et al labeled pericytes with antibodies against platelet-derived growth factor receptor beta and NG2/CSPG4, and outlined the pericyte basement membrane with fluorescently-tagged isolectin B4 [129].
Purkinje cells are a subtype of neuronal cells located in cerebellar cortex. Specific markers of Purkinje cells include cGMP-dependent protein kinase [130], guanosine 3':5'-phosphate-dependent protein kinase [131], zebrin I and zebrin II - Purkinje cell-specific markers [132-134], Car8 [135], HFB-16 (KIAA0864 Protein) [136], inositol 1, 4, 5-triphosphate receptors (IP3R) [137-139].
Pyramidal cells are neurons located in several different regions of central nervous system such as cerebral cortex, hippocampus and amygdala. They are suggested to play an important role in cognitive functions. Specific markers of pyramidal cells include CaMK (calcium/calmodulin-dependent protein kinase II, CaMKII) [140], neurogranin/RC3 [141, 142], SMI-32 [143-145], MATH-2 [146], SCIP [146, 147], Emx1 [148].
Retinal ganglion cells are the output neurons in retina. Ganglion cells acquire information about the visual world and transfer it through optical nerve to brain visual centers. Specific markers of retinal ganglion cells include RBPMS [149], NGF, NSCL2 [150], PKC, Hu, and Brn3b [151].
Glial cells are the cells located in the nervous system which provide protection and nutrition for the neurons, regulate migration of neurons in early development, communications between neurons and neurotransmitter release. Schwann cells are the main glial cells of the peripheral nervous system. Schwann cells wrap themselves around neurve axons. In addition, Schwann cells play an important role in removing debris and in the regrowth of nerve axons. Specific markers for identification of Schwann cells are S-100 [152], myelin basic protein (MBP) [153] and myelin protein zero (MPZ).
Sertoli cell have an important role in the mechanisms of spermatogenesis. In addition, Sertoli cell control the transport of hormones into the seminiferous tubules. Specific markers of Sertoli cells include ABP (androgen-binding protein) [154], Dhh (Desert hedgehog) [155], GATA-1 [156].
Y Shwartz et al labelled the arrector pili muscle with integrin alpha 8 or smooth muscle actin [157]. H Hu et al stained smooth muscle cells in human mature arteriovenous fistulae with the Abcam antibody against alpha actin ( ab5694) [30].
Cell type | Protein | Detail | Top three suppliers |
---|---|---|---|
Cardiomyocytes | PCM1 | cardiomyocyte nucleus [158] | Abcam ab154142 (3), Santa Cruz Biotechnology sc-398365 (2), Atlas Antibodies AMAb90565 (1) |
TNNT2 cardiac troponin T | cardiomyocytes [99] | Invitrogen MA5-12960 (156), Abcam ab8295 (29), Developmental Studies Hybridoma Bank CT3 (10) | |
Enterocytes | aldolase B | cytoplasmic | Abcam ab75751 (2) |
Fibroblasts | fibronectin | [106] | BD Biosciences 610077 (45), Abcam ab6328 (20), Invitrogen MA5-11981 (19) |
vimentin | heart [105], blood vessels [159] | Cell Signaling Technology 5741 (244), Invitrogen MA5-11883 (218), Abcam ab92547 (115) | |
Germ cells | AP-2gamma | gonocytes and seminomatous germ cell tumors [160, 161] | Santa Cruz Biotechnology sc-12762 (2), Abcam ab110635 (1) |
DAZ-like 1/DAZL1 | male and female gonads [110] | Bio-Rad MCA2336 (7), LifeSpan Biosciences LS-C188293 (1) | |
EGFR | syncytiotrophoblastic cells in testicular germ cell tumors [162] | Cell Signaling Technology 4267 (119), Invitrogen MA1-12693 (45), Abcam ab52894 (35) | |
MAGE-A4 | testicular tumors [163] | Santa Cruz Biotechnology sc-20034 (2) | |
OCT3/4 | germ cell tumors, such as gonadoblastoma and carcinoma in situ, invasive embryonal carcinoma and seminomatous tumors [164] | Santa Cruz Biotechnology sc-5279 (259), Cell Signaling Technology 2840 (35), BD Biosciences 611203 (14) | |
VASA | both normal and malignant human germ cells [111] | Abcam ab27591 (10), Cell Signaling Technology 8761 (4) | |
Granulosa cells | AMH/anti-mullerian hormone | a marker of sertoli- and granulosa-cell origin in gonadal tumors [115] | Bio-Rad MCA2246 (2), Santa Cruz Biotechnology sc-166752 (2), Abcam ab24542 (1) |
chZPC | a specific granulosa marker in the chicken [165] | Abcam ab171968 (1) | |
fibronectin | a marker of granulosa cell cytodifferentiation [120] | BD Biosciences 610077 (45), Abcam ab6328 (20), Invitrogen MA5-11981 (19) | |
MCAM/melanoma cell adhesion molecule/CD146 | [119] | BD Biosciences 550315 (8), Santa Cruz Biotechnology sc-18837 (5), Abcam ab75769 (5) | |
Hepatocytes | ASGR1 | BD Biosciences 563654 (3), Santa Cruz Biotechnology sc-52623 (1), Invitrogen MA1-40244 (1) | |
HP/haptoglobin | Abcam ab13429 (2), Santa Cruz Biotechnology sc-365396 (1), Abfrontier LF-MA0158 (1) | ||
Jurkat cells | CD3/CD3E | cell surface [121] | Invitrogen MA1-90582 (291), BD Biosciences 339186 (94), BioLegend 300402 (42) |
CD45 | cell surface [121] | BioLegend 103202 (149), Invitrogen 14-0452-86 (137), BD Biosciences 560777 (68) | |
IL-2 | secreted | BioLegend 500301 (17), BD Biosciences 554562 (16), Invitrogen 14-7029-81 (9) | |
Luteal cells, large | [123] | Santa Cruz Biotechnology sc-293165 (1) | |
phosphorylated Akt | [124] | Cell Signaling Technology 4060 (981), Santa Cruz Biotechnology sc-5298 (45), Abcam ab81283 (25) | |
Mast cells | CD23 | cell surface [126] | Invitrogen MA5-14572 (11), Leica Biosystems NCL-CD23-1B12 (4), BD Biosciences 550386 (3) |
CD25 | cell surface [126] | BD Biosciences 560356 (65), BioLegend 302602 (40), Invitrogen 14-0259-82 (18) | |
CD45 | cell surface [127] | BioLegend 103202 (149), Invitrogen 14-0452-86 (137), BD Biosciences 560777 (68) | |
CD117/c-Kit | cell surface [126] | Invitrogen 14-1172-85 (19), BioLegend 313201 (19), Cell Signaling Technology 3074 (18) | |
CD203c | cell surface [126] | Bio-Rad MCA1973F (2), BioLegend 324606 (1), Invitrogen MA1-10139 (1) | |
High affinity IgE receptor | cell surface [126] | BioLegend 134301 (20), Invitrogen MA1-4997 (6), Abcam ab54411 (2) | |
Purkinje cells | calbindin | cerebellar Purkinje cells [166] | MilliporeSigma C9848 (132), SWant 300 (84), Abcam ab82812 (9) |
Car8 | high levels in cerebellar Purkinje cells [135] | Santa Cruz Biotechnology sc-166626 (1) | |
cerebellin | quantifiable marker for Purkinje Cell maturation [167, 168] | Abcam ab181379 (1) | |
cGMP-dependent protein kinase | [130] | Cell Signaling Technology 3248 (3) | |
clusterin | ischaemic Purkinje cells in human brain [169] | Santa Cruz Biotechnology sc-5289 (7), Sino Biological 11297-R210 (3), Abcam ab92548 (2) | |
GAD67/67-kDa isoform of glutamic acid decarboxylase | Purkinje cell differentiation marker [170] | Abcam ab26116 (16), MilliporeSigma G5419 (3), Cell Signaling Technology 63080 (2) | |
HDAC6 | expressed by most neurons but is abundant in cerebellar Purkinje cells [171] | Cell Signaling Technology 7558 (9), Santa Cruz Biotechnology sc-28386 (3), Abcam ab253033 (1) | |
Inositol 1, 4, 5-triphosphate receptors/IP3R | [137-139] | Cell Signaling Technology 8568 (3), Santa Cruz Biotechnology sc-377518 (1), Abcam ab108517 (1) | |
NMDA-NR1/NMDA-R1 receptor subtype | adult cerebellar Purkinje cell [172] | Invitrogen 32-0500 (18), Cell Signaling Technology 5704 (9), Synaptic Systems 114 011 (7) | |
PCA-1/PCA-2 | cytoplasm of Purkinje cells [173, 174, 174, 175] | Santa Cruz Biotechnology sc-166649 (1) | |
PMCA/plasma membrane calcium pump | more distal dendrites of the Purkinje cells [176] | Invitrogen MA3-914 (58), Santa Cruz Biotechnology sc-20028 (4), LifeSpan Biosciences LS-C87407 (1) | |
PMCA2/plasma membrane Ca(2+)-transport ATPase-2 | [177, 178] | Invitrogen MA3-914 (58) | |
SERCA | [179] | Abnova H00000489-M01 (1) | |
Paneth cells | lysozyme | cytoplasmic | Abcam ab108508 (6), Invitrogen GIC207 (1) |
Pyramidal cells | CaMK2 alpha | [140] | Invitrogen MA1-047 (45), Abcam ab22609 (16), Cell Signaling Technology 12716 (14) |
MAP2 | pyramidal cell dendrites [180-182] | MilliporeSigma M4403 (94), Invitrogen MA5-12823 (23), Abcam ab11267 (23) | |
mGluR5 | major postsynaptic mGluR expressed in CA1 pyramidal neurons [183, 184] | Abcam ab76316 (5) | |
PSD-95 | dendrites of pyramidal neurons [186] | Invitrogen MA1-045 (209), Neuromab 75-028 (93), Cell Signaling Technology 3450 (40) | |
SCIP | hippocampal pyramidal cell marker [146, 147] | Abcam ab126746 (3) | |
SMI-32/CD3epsilon | [143-145] | Invitrogen MA1-90582 (291), BD Biosciences 339186 (94), BioLegend 300402 (42) | |
Retinal ganglion cells | NSCL2 | Santa Cruz Biotechnology sc-735 (4) | |
PKC alpha | Santa Cruz Biotechnology sc-8393 (21), Abcam ab32376 (13), Novus Biologicals NB600-201 (13) | ||
POU4F1 / BRN3A | Santa Cruz Biotechnology sc-31984 (23), Abcam ab81213 (2) | ||
RBPMS | Santa Cruz Biotechnology sc-293285 (1) | ||
Schwann cells | glial fibrillary acidic protein/GFAP | non-myelinating Schwann cells | MilliporeSigma G3893 (234), Invitrogen 13-0300 (96), Cell Signaling Technology 3670 (66) |
myelin basic protein/MBP | myelinating Schwann cells | Abcam ab7349 (42), BioLegend 808401 (23), Santa Cruz Biotechnology sc-271524 (8) | |
S100A1 | Invitrogen MA5-12969 (57), Abcam ab4066 (16), Biogenex MU058-5UC (3) | ||
Sertoli cells | ABP/androgen-binding protein | a functional marker of Sertoli cells [154, 187, 188] | Santa Cruz Biotechnology sc-377031 (1) |
calretinin | immature Sertoli cells [189] | SWant 6B3 (45), Invitrogen MA1-39562 (9), BD Biosciences 610908 (8) | |
clusterin | rat Sertoli cells [190, 191] | Santa Cruz Biotechnology sc-5289 (7), Sino Biological 11297-R210 (3), Abcam ab92548 (2) | |
cytokeratin 18 | Sertoli cells in prenatal and prebubertal periods [189, 192, 193] | Invitrogen MA1-82041 (104), Abcam ab668 (21), MilliporeSigma C2562 (21) | |
GATA-1 | Sertoli cells at specific seminiferous tubule stages [156] | Santa Cruz Biotechnology sc-265 (13), Cell Signaling Technology 3535 (1) | |
GATA-4 | fetal Sertoli cells in the seminiferous cords [194-198] | Santa Cruz Biotechnology sc-25310 (36), Invitrogen 14-9980-80 (2) | |
MIS/mMullerian inhibiting substance | fetal Sertoli cells [199] | Bio-Rad MCA2246 (2), Santa Cruz Biotechnology sc-166752 (2), Abcam ab24542 (1) | |
SCF/stem cell factor | produced by Sertoli cells [200-204] | Abcam ab52603 (5), Santa Cruz Biotechnology sc-13126 (1) | |
Sox9 | regulates the differentiation of Sertoli cells in the testis [207-210] | Abcam ab185966 (15), Santa Cruz Biotechnology sc-166505 (3), Abnova H00006662-M04 (1) | |
vimentin | mature Sertoli cells [211, 212] | Cell Signaling Technology 5741 (244), Invitrogen MA5-11883 (218), Abcam ab92547 (115) |
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- gene
- human ABAT
- human ABCB1
- human ABCG2
- human ACKR1
- human ADAM19
- human ALDOC
- human AMH
- human ASGR1
- human ATP2A3
- human ATP2B1
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- human CD1a
- human CD2
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- human CD244
- human CD25
- human CD27
- human CD32
- human CD33
- human CD34
- human CD38
- human CD3E
- human CD4
- human CD43
- human CD44
- human CD45
- human CD55
- human CD56
- human CD57
- human CD59
- human CD68
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- human CD86
- human CD9
- human CD90
- human CD93
- human CD94
- human CLEC4C
- human CLIP1
- human CLU
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- human FCER1A
- human FEZF2
- human FGFR3
- human FLT3
- human Flt-1
- human GAD1
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- human GATA3
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- human HDAC6
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- human IL3
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- human IRF7
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- human ITGA1
- human ITGA4
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- human ITPR1
- human KIR3DL1
- human KITLG
- human KLRB1
- human KLRF1
- human KRT10
- human KRT15
- human KRT16
- human KRT2
- human KRT3
- human KRT5
- human KRT6A
- human KRT8
- human LAMP-1
- human LAMP3
- human LFA-1
- human LHCGR
- human LIN28A
- human LSECtin
- human LTA
- human LY75
- human LYVE 1
- human MAGEA4
- human MAP2
- human MBP
- human MCAM
- human MPL
- human MPRIP
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- human MUC1
- human NCR1
- human NECTIN1
- human NEUROD6
- human NGF
- human NKG2A
- human NKG7
- human NR1
- human OMP
- human OR3A3
- human Oct4
- human PC 1
- human PCM1
- human PCP4
- human PDE1B
- human PDE5A
- human PDE9A
- human PDGFR beta
- human PECAM 1
- human PKC alpha
- human PNPLA2
- human POU3F1
- human POU4F1
- human PRKG1
- human PSD-95
- human PTPRG
- human Prox1
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- human RBPMS
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- human aldolase B
- human apelin
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- human calbindin
- human calretinin
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- human cystatin C
- human cytokeratin 14
- human cytokeratin 18
- human cytokeratin 7
- human cytokeratin
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- human endomucin
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- human interleukin 2
- human interleukin 5
- human leptin
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- human lysozyme
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- human podoplanin
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method- 3D Cell Culture: A Review
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- Incorporating Unnatural Amino Acids into Recombinant Proteins in Living Cells
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- Macrophage Markers
- Microglia Markers
- Neuronal Cell Markers
- Stem Cell Markers
- Stem Cell Research Using Mouse Models
- Stem Cells
- Subcellular Fractionation
- T Cell Markers and B Cell Markers
- The Cell Cycle Analysis